Facts About attune flow cytometer Revealed
Facts About attune flow cytometer Revealed
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Information-enriched characterization of mobile populations. Morphological information from pictures can include to the richness of flow cytometry facts. Such as, the figure exhibits an or else conventional apoptosis assay making use of Annexin V and PI, introducing cell imaging to characterize cells in Just about every populace to expose morphologically distinctive features. These insights could not have already been attained from multiplex staining by itself.
As samples are obtained about the Attune CytPix Flow Cytometer, the high-velocity brightfield digital camera captures and retailers photographs of detected activities at a level around 6,000 visuals for each next, depending upon the flow rate and impression dimensions. For larger versatility, Attune Cytometric Software program helps you to alter the picture capture frequency as desired and select a particular gate for picture capture, if needed.
(B) Uncooked 264.seven macrophages will often be annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The number of singlets obtainable for Examination raises when the person trains and employs a more accurate product.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a high-pace brightfield camera for simultaneous high throughput flow cytometry and large resolution brightfield imaging.
"An image isn't really value 1000 dots, but it surely seriously helps to see photographs. It is great to determine pictures that correlate with staining. By way of example, AnnexinV-good cells which are morphologically diverse than healthful cells, or CD14+ monocytes which can be greater and much more textured than CD3+/CD4+/CD14- lymphocytes.
Furthermore, it can be illuminating to determine the quantity of doublets slip by conventional singlet gates, and wonderful to confirm the position of unpredicted double-positive occasions in my Assessment."
Pair your Attune flow cytometer with the autosampler for enhanced workflow efficiency, and add robotic integration for maximum throughput.
Our scientists will design and style panels for you as many times you will need—free of charge, no product order needed.
Early signs of this contamination had in the beginning been dismissed as particles. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological features to find out attention-grabbing subpopulations that might not be apparent from flow cytometry knowledge by yourself.
Maybe most importantly, these situations consist of cells of Plainly unique phenotypes which may lead to incorrect conclusions regarding double good functions (particularly in uncommon populations).
Seem waves empower precise mobile alignment, leading to Fantastic knowledge top quality and ~10X a lot quicker sample flow rates.
We Beforehand shown the strength of imaging Automobile-T/Ramos cell interactions. Allow’s have a look at just the inhabitants of best interest, the double optimistic functions, to learn more. We are able to now use extended morphological parameters (listed here, circularity and skewness of depth) to further more take a look at the functions of such populations and refine gating on these occasions, expanding information robustness.
Created-in dimensionality reduction tools enhanced resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to substantiate expected inhabitants resolution in lysed whole human blood. Person can see occasions from Just about every cluster for Visible confirmation of cell phenotypes.
Versatile payment program from Agilent Fiscal Alternatives let you have the crucial instrumentation you require for fast task, or to develop your lab.
At the center of every Attune Flow Cytometer lies our innovative acoustic concentrating technology, which utilizes seem waves to meticulously align cells throughout the flow cell. This revolutionary strategy surpasses common hydrodynamic strategies, causing quicker processing speeds, Remarkable data high quality, and enhanced sample throughput.
Because the mobile line had Formerly been analyzed on the Attune CytPix Flow Cytometer, the scientists went again to the images and ended up ready to document the microbial infection at the least five times previously.
The Attune CytPix Flow Cytometer lets users to visualize samples for the duration of acquisition, confirm the precision of gating approaches, and guidance benefits with check here reliable documentation for publication reasons.
What appeared to be a homogeneous population is in fact much more numerous—an insight that should be thought of when interpreting final results.